Development and utilization of analytical methods for rapid GM detection in processed food products: a case study for regulatory requirement

Genetically modified (GM) food crops for desired traits have been approved in some of the countries. The approval status a GM event varies from country to country. In India, Bt cotton is only crop. So far, no crop has got regulatory country, which may be considered as unauthorized (UGM) Indian context. entry UGM events needs checked imported consignments well marketplace. present study, screening elements were identified based on genetically organism (GMO) matrix developed decision support system 22 globally. Three sets multiplex PCR assays and validated detection products: triplex targeting control [Cauliflower Mosaic Virus 35S promoter (P-35S), Figwort (P-FMV), nopaline synthase terminator (T-nos)], marker genes [aminoglycoside-3’-adenyltransferase (aadA), neomycin phosphotransferase (nptII), phosphinothricin-N-acetyltransferase (pat)] duplex (cry1Ab/Ac cry2Ab2). Limit (LOD) ranged 0.5–0.01% different targets, compliance with labelling threshold many utilized check apple maize products along an additional test ctp2-cp4epsps herbicide tolerance maize. Based tests conducted, none these samples found positive targets screened. These procedures could efficiently employed part GMO testing trace contamination, if any, both domestic products.